Skip to main content
. 2020 Jul 15;18(7):365. doi: 10.3390/md18070365

Figure 5.

Figure 5

Changes in the levels of mRNAs encoding c-MET, PI3KC2β, PLCγ1, Cdc42, and ROCK1 in H. pylori-infected AGS cells, with and without ASTX pretreatment, determined by real-time PCR. AGS cells were pretreated with vehicle (DMSO) or 5 μM ASTX for 3 h and then incubated with H. pylori at a 1:50 cell ratio for 1 h. The relative amounts of c-MET, PI3KC2β, PLCγ1, Cdc42, and ROCK1 mRNA in AGS cells were measured by real-time PCR. The mRNA levels were normalized to that of β-actin (A) or 18S rRNA (B). All values are expressed as the mean ± S.E. For each experiment, the number of each group was three (n = 3 per each group). A Student’s t-test was used for statistical analysis. * p < 0.05 vs. uninfected cells; + p < 0.05 vs. H. pylori-infected cells without ASTX treatment.