Figure 1.

Effect of ethyl acetate extract of Enteromorpha prolifera (EAEP) on glutamate-induced cytotoxicity and reduction in reactive oxygen species (ROS) in hippocampal neuronal cells (HT-22). HT-22 cells, seeded on 96-well-plates and incubated for 24 h, were treated with or without EAEP (0–100 µg/mL) for 30 min before glutamate challenge (5 mM). After 12 h, cell viability and intracellular ROS levels were estimated as described in the Materials and Methods. (A) Cell viability. (B) ROS level. Data represent the mean ± SEM values in triplicate; ** p < 0.01 versus glutamate-treated group. − denotes absence of EAEP.