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. 2020 Jul 19;18(7):372. doi: 10.3390/md18070372

Figure 3.

Figure 3

Treatment with ethyl acetate extract of Enteromorpha prolifera (EAEP) activates the expression of antioxidant enzymes via Akt/Nrf2 pathway. (A) Immunoblotting for protein expression related with Akt/Nrf2 pathway, (B) relative density of p-Akt, nuclear and cytosolic Nrf2, (C) NQO-1, GLCL, and HO-1. HT-22 cells were seeded on a 60-mm dish, and incubated for 24 h. The cells were challenged with glutamate after pre-incubation with or without EAEP (0–100 µg/mg) for 30 min. After 12 h, the expression of p-Akt, nuclear and cytosolic Nrf2, HO-1, NQO-1, GCLC or β-actin was examined as described in the Materials and Methods. The data were based on three independent experiments. ** p < 0.01 versus glutamate-treated group. − denotes absence of EAEP.