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. 2020 Jul 19;18(7):372. doi: 10.3390/md18070372

Figure 5.

Figure 5

Treatment with K252a or MK-2206 inhibits neuroprotective activity of ethyl acetate extract of Enteromorpha prolifera (EAEP). HT-22 cells were pre-incubated with or without EAEP in combination with K252a or MK-2206 for 30 min before glutamate challenge. After 12 h, the cell viability and ROS levels were measured as described in the Materials and Methods. (A,C) Cell viability; and (B,D) ROS levels. Data represent the mean ± SEM values based on quintuple determinations. ** p < 0.01 versus glutamate-treated group; ## p < 0.01 versus EAEP with glutamate-treated group. − absent, + present.