Treatment with K252a or MK-2206 inhibits neuroprotective effects of ethyl acetate extract of Enteromorpha prolifera (EAEP). (A) Immunoblotting for protein expression related with BDNF/TrkB pathway, and (B) relative density of BDNF and p-TrkB. HT-22 cells were pre-incubated with or without EAEP in combination with K252a or MK-2206 for 30 min before glutamate challenge. After 12 h, the expression of BDNF, p-TrkB, or β-actin was examined as described in the Materials and Methods. The data were obtained from three independent experiments. ** p < 0.01 versus glutamate-treated group; ##
p < 0.01 versus EAEP with glutamate-treated group. − is absence, + is present.