Figure 1.

Effects of palmitate and resveratrol on serine (ser³⁰⁷ and ser^636/639) phosphorylation and expression of IRS-1. Whole-cell lysates from L6 myotubes treated without (control, C) or with 0.4 mM palmitate (P), in the absence or the presence 25 µM resveratrol (RSV) were prepared, resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and immunoblotted for phosphorylated ser³⁰⁷, ser^636/639, total IRS-l or β-actin. A representative immunoblot is shown (A). The densitometry of the bands, expressed in arbitrary units, was measured using the Scion software. The data are the mean ± SE of three separate experiments presented as percent of control (B) (**p < 0.01, ***p < 0.001 vs. control; #p < 0.05, ##p < 0.01 as indicated).