Figure 7.

Effects of palmitate and resveratrol on insulin-stimulated Akt phosphorylation/expression. L6 myotubes were treated without (control, C) or with 0.4 mM palmitate for 16 h (P), in the absence or the presence of 25 µM resveratrol (RSV), followed by acute stimulation with 100 nM insulin for 30 min (I). Proteins (20 µg) in whole-cell lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and immunoblotted for phosphorylated ser⁴⁷³Akt, total Akt, or β-actin. A representative immunoblot is shown (A). The densitometry of the bands, expressed in arbitrary units, was measured using the Scion software. The data are the mean ± SE of three separate experiments presented as percent of control (B). The data presented as the ratio of phosphorylated Akt to total Akt are shown (C) (*p < 0.05, **p < 0.01 vs. control; # p < 0.05, ## p < 0.01 as indicated).