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. 2020 Jul 16;21(14):5020. doi: 10.3390/ijms21145020

Figure 1.

Figure 1

Mouse genotyping. (a) β-globin gene in mice was amplified by PCR and used as control. β-globin amplification (494 bp fragment) was observed in all mice; (b) The presence of the HPV16 early region was assessed by the amplification of the HPV16 E7 gene (157 bp fragment). As expected, amplification was not observed in wild-type mice (Groups 1 and 2 represented by Samples 1 and 2, respectively) but only in HPV+ animals (Groups 3 and 4 represented by Samples 3 and 4, respectively). MW: molecular weight marker (GeneRuler 50 bp, Thermo Scientific, Waltham, MA, USA). NC: negative control.