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Experimental design timeline. Weekly DMBA application (0.031 mg/animal/week in 4 μL of DMSO) started at 9–11 weeks of age, lasting for 17 weeks. Groups 2 and 4 received DMBA, while Groups 1 and 3 received DMSO only (the vehicle). Animals were sacrificed at 31–33 weeks of age for sample collection. wo: weeks old.
