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. 2020 Jul 17;21(14):5067. doi: 10.3390/ijms21145067

Figure 4.

Figure 4

Acute application of ouabain (Oua, 10 nM) to the basolateral side does not alter transepithelial resistance (TER) of rat jejunum and colon (a) as well as claudin-1 expression in IPEC-J2 cells (b). (a) Vertical arrow indicates the time of ouabain addition. Each data point represents the mean of measurements from 4–8 intestine fragments obtained from 4 rats. (b) Western blot analysis of claudin-1 (CLDN-1) expression (n = 6 for each group); upper panel shows representative immunoblots. After 19 days of culture, non-treated IPEC-J2 cells were incubated in the absence (control medium) or in the presence of 10 nM ouabain added for 240 min with subsequent Western blot analysis. Original images for Western blots using Stain-Free gels as a loading control are shown in Supplemental Materials. The number of symbols corresponds to the number of samples. One-way ANOVA with Dunnett correction.