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. 2020 Jul 17;21(14):5067. doi: 10.3390/ijms21145067

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chronic exposure to ouabain (Oua) altered claudin expression in rat brain frontal lobes. Rats were intraperitoneally injected with ouabain (1 µg/kg) for 4 days. (a) Representative immunoblots. (b) Western blot analysis of claudin (CLDN) and occludin expression (n = 4 for each group); Original images for Western blots using Stain-Free gels as a loading control are shown in Supplemental Materials. The number of symbols corresponds to the number of samples. One-way ANOVA with Dunnett correction: * p < 0.05 compared with the control (vehicle treated group).