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. 2020 Jul 21;21(14):5166. doi: 10.3390/ijms21145166

Figure 6.

Figure 6

LfP regulate cytokine secretion from murine bone marrow macrophages. Bone marrow macrophages from unchallenged mice were treated with FKE or FKD peptides (1–1000 µM) for 24 h, followed by LPS exposure for additional 24 h. Next, culture media was collected and its cytokine content was determined by standard ELISA for TNF-α (A), IL-6 (B), or IL-10 (C). Results are representative from two experiments (n = 4 mice for each experiment) and presented as mean ± SD with biological and analytical duplicates for each treatment. * and # indicate p < 0.05 compared to treatment with LPS alone or with other peptide, respectively. All treatments were statistically different from untreated cells (ANOVA).