Figure 1.

Standard curve of cycle threshold (Ct) values were obtained on the basis of efficiency and correlation of coefficient (R²) in serial dilution series recombinant plasmids (C. longa and Z. mays) using species-specific primer sets. The x-axis represents log number of plasmids and the y-axis represents means of Ct value ± SD. (A) C. longa targeting primer sets (CL_matK, CL_atpF, and CL_ycf2). Green dots represent serial dilution series of recombinant plasmids (107–103) containing C. longa specific target gene (matK, atpF and ycf2) sequences; (B) Z. mays targeting primer sets (ZM_matK, ZM_atpF, and ZM_ycf2). Blue dots represent serial dilution series of recombinant plasmids (107–103) containing Z. mays specific target gene (matK, atpF and ycf2) sequences. The real-time PCRs were carried out in triplicate (n = 3).