Table 3.

Results of the blind mixture test for evaluating the reliability of the developed primer sets.

No.	Ingredient		PACa	Eb (%)	Z. mays Specific Primer Ct ± SD			A/D
	C. longa (%)	Z. mays (%)			ZM_matK	ZM_atpF	ZM_ycf2
1	99	1	13.57 ± 0.04	0.5–1.5	25.02 ± 0.06	25.61 ± 0.20	26.44 ± 0.21	Ad
2	98	2	14.73 ± 0.09	1–5	25.09 ± 0.02	24.78 ± 0.12	22.8 ± 0.18	A
3	100	0	14.03 ± 0.01	NDc	31.51 ± 0.15	31.32 ± 0.26	35.15 ± 0.05	A
4	98	2	14.31 ± 0.01	1–5	24.40 ± 0.01	24.75 ± 0.15	23.92 ± 0.09	A
5	95	5	14.05 ± 0.01	1–5	23.72 ± 0.09	24.34 ± 0.09	21.94 ± 0.22	A
6	97	3	14.11 ± 0.06	1–5	23.45 ± 0.05	24.90 ± 0.07	22.97 ± 0.25	A
7	99.5	0.5	14.21 ± 0.02	0.5–1.5	25.09 ± 0.05	26.95 ± 0.07	26.34 ± 0.15	A
8	98.5	1.5	14.12 ± 0.05	0.5–1.5	25.59 ± 0.03	26.32 ± 0.10	24.69 ± 0.19	A
9	100	0	14.33 ± 0.08	ND	31.67 ± 0.20	31.01 ± 0.80	34.89 ± 0.10	A
10	98.5	1.5	14.23 ± 0.03	0.3–2	25.13 ± 0.09	25.69 ± 0.10	24.21 ± 0.02	A
11	99.5	0.5	14.27 ± 0.01	0.1–1	26.06 ± 0.09	27.08 ± 0.10	26.19 ± 0.09	A
12	100	0	13.61 ± 0.12	ND	31.33 ± 0.28	30.98 ± 0.11	34.46 ± 0.26	A
13	97	3	14.31 ± 0.10	1–5	24.97 ± 0.09	24.37 ± 0.07	23.86 ± 0.13	A
14	98	2	13.22 ± 0.07	1–5	24.19 ± 0.09	26.14 ± 0.12	23.22 ± 0.09	A
15	96	4	13.33 ± 0.06	1–5	23.92 ± 0.15	24.26 ± 0.03	22.72 ± 0.27	A
16	99	1	14.26 ± 0.05	0.1–1	25.75 ± 0.10	26.75 ± 0.14	25.11 ± 0.10	A
17	93	7	13.95 ± 0.07	5–10	22.11 ± 0.11	23.99 ± 0.13	21.72 ± 0.11	A
18	90	10	13.74 ± 0.02	10–15	22.11 ± 0.10	21.99 ± 0.18	21.23 ± 0.13	A
19	100	0	16.21 ± 0.07	ND	30.82 ± 0.20	31.09 ± 0.01	34.06 ± 0.56	A
20	97	3	13.27 ± 0.1	1–5	23.96 ± 0.05	24.28 ± 0.10	23.22 ± 0.12	A

^a Positive amplification control (18s rRNA); ^b, expected ratio of the Z. mays; ^C, not detected; ^d accordance.