FIGURE 5.
Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa.; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H2O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com]