Table 1.
Solvent gradient elution program used for the elution of sugars. Negative values represent equilibration times.
| Time (min) | %A | %B | Flow Rate (mL min−1) |
|---|---|---|---|
| −35.000 | 100.00 | 0.000 | 0.300 |
| −30.100 | 100.00 | 0.000 | 0.300 |
| −30.000 | 100.00 | 0.000 | 0.150 |
| −11.000 | 100.00 | 0.000 | 0.150 |
| −10.000 | 100.00 | 0.000 | 0.300 |
| 0.000 | 100.00 | 0.000 | 0.300 |
| 10.000 | 100.00 | 0.000 | 0.300 |
| 35.000 | 0.000 | 100.00 | 0.300 |
| 45.000 | 0.000 | 100.00 | 0.300 |
Statistical analysis, i.e., linear regression and intra- and interday repeatability, was performed with Chromeleon 7.2.8 software and Microsoft Office Excel 365 (Redmond, WA, USA).