Fig. 4.

HMF triggers apoptosis in NCI-H1975 cells. a NCI-H1975 cells were treated with indicated concentrations of HMF (31.3–250 μg/mL) for 48 h, respectively, stained with Annexin V-fluorescein isothiocyanate (Annexin V-FITC)/PI, and determined by flow cytometry. b The bar graph depicts the percentage of total apoptotic cell percentage of NCI-H1975 cells in the indicated concentration of HMF. All experiments were performed in three replicates. * P < 0.05 was considered significant