Effects of different concentrations of ethephon pretreatment on the infected grape with B. cinerea. (A) The development of B. cinerea on the surface of ‘Kyoho’ grape fruits in pretreated samples with different concentrations of ethephon treatment (100, 200, 300, 400, 500, 600, 700, 800, 900, 1000 mg/L) for 24 h and then inoculation with B. cinerea. Samples were monitored at 1, 48, and 72 h after botrytis infection. (B) The B. cinerea mycelium grew on the surface of the grape fruit and was observed by electron microscope at 72 h. (C) Fruit disease incidence and lesion diameter changes were calculated. Values are means ± SD of twenty biological replicates. ** Significant differences compared with the control (water-treated fruits) sample at p < 0.01, using Student’s test.