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. 2020 Jun 29;12(7):430. doi: 10.3390/toxins12070430

Table 1.

Insecticidal activity of Cry and Cyt proteins against coleopteran pests.

Crystal Type Toxin Target Insect Activity (a) LC50 (b) Reference
Scientific Name Family
Cry1Aa Anoplophora glabripennis Cerambycidae N [86]
Apriona germari Cerambycidae N [87]
Epilachna varivestis Coccinellidae A [88]
Tribolium castaneum Tenebrionidae LA [89]
Cry1Ab Diabrotica undecimpuntata Chrysomelidae N [90]
Leptinotarsa decemlineata Chrysomelidae N [90]
Phyllotreta armoraciae Chrysomelidae N [90]
Adalia bipunctata Coccinellidae N [91]
Atheta coriaria Coccinellidae N [91]
Cryptolaemus montrouzieri Coccinellidae N [91]
Harmonia axyridis Coccinellidae N [92]
Anthonomus grandis Curculionidae N [90]
Hypera postica Curculionidae N [90]
Popillia japonica Scarabaeidae N [90]
Cry1A c Diabrotica undecimpuntata Chrysomelidae N [90]
Leptinotarsa decemlineata Chrysomelidae N [90]
Phyllotreta armoraciae Chrysomelidae N [90]
Hippodamia convergens Coccinellidae N [93]
Anthonomus grandis Curculionidae N [90]
Hypera postica Curculionidae N [90,94]
Haptoncus luteolus Nitidulidae N [95]
Tribolium castaneum Tenebrionidae N [96]
Popillia japonica Scarabaeidae N [90]
Cry1Ah Propylea japónica Coccinellidae N [97]
Cry1Aj Harmonia axyridis Coccinellidae N [92]
Cry1Ba Anoplophora glabripennis Cerambycidae N [86]
Acanthoscelides obtectus Chrysomelidae A [75]
Chrysomela scripta F Chrysomelidae A 1.8 // 5.9 [71,72]
Leptinotarsa decemlineata Chrysomelidae A 1050 // 142 [71,73]
Phaedon cochleariae Chrysomelidae N [98]
Anthonomus grandis Curculionidae A 305.32 [74]
Asymmathetes vulcanorum Curculionidae N [99]
Hypothenemus hampei Curculionidae A [100]
Tribolium castaneum Tenebrionidae N [89]
Cry1Ca Tribolium castaneum Tenebrionidae N [89]
Cry1Da Tribolium castaneum Tenebrionidae N [89]
Cry1Ea Tribolium castaneum Tenebrionidae N [89]
Cry1Fa Cryptolestes pusillus Laemophloeidae N [17]
Tribolium castaneum Tenebrionidae N [17]
Cry1Fb Tribolium castaneum Tenebrionidae N [89]
Cry1Ia Acanthoscelides obtectus Chrysomelidae A [75]
Agelastica coerulea Chrysomelidae N [101,102]
Diabrotica undecimpuntata Chrysomelidae N [103]
Leptinotarsa decemlineata Chrysomelidae A 33.7 // 10 [73,104]
Phaedom brassicae Chrysomelidae N [101]
Anthonomus grandis Curculionidae A 21.5 // 230 [76,105]
Asymmathetes vulcanorum Curculionidae N [99]
Tenebrio molitor Tenebrionidae N [106]
Tribolium castaneum Tenebrionidae N [89]
Cry1Ib Phaedom brassicae Chrysomelidae N [101]
Agelastica coerulea Chrysomelidae N [101]
Cry1Id Agelastica coerulea Chrysomelidae N [102]
Cry1Ie Ceratoma trifurcata Chrysomelidae N [107]
Pyrrhalta aenescens Chrysomelidae N [108]
Cry1Jb Diabrotica undecimpuntata Chrysomelidae N [109]
Leptinotarsa decemlineata Chrysomelidae N [109]
Cry2Aa Diabrotica undecimpuntata Chrysomelidae N [93]
Diabrotica virgifera Chrysomelidae N [93]
Leptinotarsa decemlineata Chrysomelidae N [93]
Hippodamia convergens Coccinellidae N [93]
Anthonomus grandis Curculionidae N [93]
Cry2Ab Propylea japonica Coccinellidae N [97]
Haptoncus luteolus Nitidulidae N [95]
Cry3Aa Rhyzophertha dominica Bostrichidae A 1.17 μg/mg [110]
Cylas brunneus Brentidae A 1.88 μg/g [56]
Cylas puncticollis Brentidae A 1.99 μg/g [56]
Apriona germari Cerambycidae A [94,111]
Acanthoscelides obtectus Chrysomelidae A [75]
Agelastica alni Chrysomelidae A [112]
Brontispa longissimi Chrysomelidae A 0.475 mg/mL [113]
Chrysomela tremulae Chrysomelidae A [114]
Chrysomela scripta F Chrysomelidae A [115]
Chrysomela scripta F Chrysomelidae A 2.22 // 1.8 [71,72]
Colaphellus bowringi Chrysomelidae A 2.68 // 1.33 [116,117]
Crioceris quaturdicerumpunctata Chrysomelidae A 3.82 [117]
Diabrotica undecimpuntata Chrysomelidae N [90,118]
Diabrotica virgifera Chrysomelidae N [118,119]
Leptinotarsa decemlineata Chrysomelidae A 1.84 // 3.56 [73,118]
Phaedom brassicae Chrysomelidae A 1.11 [117]
Phaedon cochleariae Chrysomelidae A [120]
Phyllotreta armoraciae Chrysomelidae N [90]
Plagiodera versicolora Chrysomelidae A 1.13 // 3.09 [18]
Pyrrhalta aenescens Chrysomelidae A 0.22 mg/ml [121]
Pyrrhalta luteola Chrysomelidae A 0.12 μg/cm2 [49]
Adalia bipunctata Coccinellidae N [91]
Atheta coriaria Coccinellidae N [91]
Cryptolaemus montrouzieri Coccinellidae N [91]
Epilachna varivestis Coccinellidae A [88]
Anthonomus grandis Curculionidae N [90]
Asymmathetes vulcanorum Curculionidae N [99]
Hypera postica Curculionidae N [90]
Hypothenemus hampei Curculionidae A [100]
Myllocerus undecimpustulatus Curculionidae A 152 ng/cm2 [122]
Premnotrypes vorax Curculionidae LA [123]
Sitophilus oryzae Curculionidae A [124]
Amphimallon solstitiale Scarabaeidae A [112]
Anomala corpulenta Scarabaeidae N [116]
Melontha melontha Scarabaeidae A [112]
Popillia japonica Scarabaeidae N [90]
Alphitobius diaperinus Tenebrionidae A 9.58 // 8 μg/cm2 [62,125]
Tribolium castaneum Tenebrionidae N [96,110]
Tribolium castaneum Tenebrionidae A 0.46 g/10 g [89]
Tenebrio molitor Tenebrionidae A 11.4 μg/larve [126]
Tenebrio molitor Tenebrionidae A [110,127]
Cry3Ba Cylas brunneus Brentidae A 1.304 μg/g [56]
Cylas puncticollis Brentidae A 1.273 μg/g [56]
Chrysomela scripta F Chrysomelidae A [115]
Diabrotica undecimpuntata Chrysomelidae A 107 ng/mm2 [128]
Leptinotarsa decemlineata Chrysomelidae A 1.35 ng/mm2 [128]
Epilachna varivestis Coccinellidae N [88]
Popillia japonica Scarabaeidae A 1 [37]
Tribolium castaneum Tenebrionidae A 1.60 g/10 g [89]
Tribolium castaneum Tenebrionidae A 13.55 mg/mL [37,96]
Cry3Bb Cylas brunneus Brentidae A 1.83 μg/g [56]
Cylas puncticollis Brentidae A 1.82 μg/g [56]
Anoplophora glabripennis Cerambycidae N [86]
Diabrotica undecimpuntata Chrysomelidae A 9.49 // 1.18 [118,129]
Diabrotica virgifera Chrysomelidae A 2.10 // 5.18 [118,129]
Leptinotarsa decemlineata Chrysomelidae A 6.86 // 6.54 [118,129]
Alphitobius diaperinus Tenebrionidae A 26.52 // 50 μg/cm2 [62,125]
Cry3Ca Cylas brunneus Brentidae A 0.69 μg/g [56]
Cylas puncticollis Brentidae A 0.57 μg/g [56]
Leptinotarsa decemlineata Chrysomelidae A 0.7 // 320.13 [130,131]
Tribolium castaneum Tenebrionidae N [96]
Cry6Aa Diabrotica virgifera Chrysomelidae A 77 µg/cm2 [66,119]
Hypera brunneipennis Curculionidae A [66]
Hypera postica Curculionidae A [66]
Cry6Ba Hypera postica Curculionidae A 280 ng/μl [94]
Cry7Aa Cylas brunneus Brentidae A 0.44 μg/g [56]
Cylas puncticollis Brentidae A 0.34 μg/g [56]
Anoplophora glabripennis Cerambycidae N [86]
Diabrotica undecimpuntata Chrysomelidae N [52]
Leptinotarsa decemlineata Chrysomelidae A 13.1 // 18.8 [52,57]
Anthonomus grandis Curculionidae N [52]
Cry7Ab Acanthoscelides obtectus Chrysomelidae A [75]
Ceratoma trifurcata Chrysomelidae N [107]
Colaphellus bowringi Chrysomelidae A 293.79 [132]
Pyrrhalta aenescens Chrysomelidae N [58]
Henosepilachna vigintioctomaculata Coccinellidae A 209 [58,133]
Anomala corpulenta Scarabaeidae N [58]
Tribolium castaneum Tenebrionidae LA [89]
Cry8Aa Leptinotarsa decemlineata Chrysomelidae A [134]
Cotinis spp Scarabaeidae A [135]
Tribolium castaneum Tenebrionidae LA [89]
Cry8Ab Holotrichia oblita Scarabaeidae A 5.72 μg/g [136]
Holotrichia parallela Scarabaeidae A 2.00 μg/g [136]
Tenebrio molitor Tenebrionidae N [136]
Cry8Ba Diabrotica virgifera Chrysomelidae A [119]
Cotinis spp Scarabaeidae A [137]
Cyclocephala borealis Scarabaeidae A [135]
Cyclocephala pasadenae Scarabaeidae A [135]
Popillia japonica Scarabaeidae A [135]
Cry8Bb Diabrotica undecimpuntata Chrysomelidae A [138]
Diabrotica virgifera Chrysomelidae A [138]
Leptinotarsa decemlineata Chrysomelidae A [138]
Cry8Ca Anoplophora glabripennis Cerambycidae N [86]
Colaphellus bowringi Chrysomelidae N [116]
Leptinotarsa decemlineata Chrysomelidae N [116]
Epilachna varivestis Coccinellidae A [88]
Anomala corpulenta Scarabaeidae A 1.75 × 10 × 108 CFU/g [116]
Anomala corpulenta Scarabaeidae A 1.6 × 10 × 108 CFU/g [139,140]
Anomala cuprea Scarabaeidae A [141]
Anomala exoleta Scarabaeidae A [142]
Holotrichia parallela Scarabaeidae A 9.24 × 10 × 108 CFU/g [140]
Popillia japonica Scarabaeidae A 12.3 μg/g [35]
Alphitobius diaperinus Tenebrionidae A 7.71 // 10 μg/cm2 [62,125]
Tribolium castaneum Tenebrionidae N [89]
Cry8Da Anomala cuprea Scarabaeidae A [143]
Anomala orientalis Scarabaeidae A [143]
Popillia japonica Scarabaeidae A 17.0 μg/g [35,143]
Cry8Db Popillia japonica Scarabaeidae A 19.6 μg/g [35]
Cry8Ea Plagiodera versicolora Chrysomelidae A [144]
Anomala corpulenta Scarabaeidae A [140]
Holotrichia parallela Scarabaeidae A 0.9 × 10 × 108 CFU/mL [59,64,144]
Popillia japonica Scarabaeidae A [144]
Tenebrio molitor Tenebrionidae N [64]
Tribolium castaneum Tenebrionidae N [64,89]
Cry8Fa Anomala corpulenta Scarabaeidae N [59]
Holotrichia oblita Scarabaeidae N [59]
Holotrichia parallela Scarabaeidae N [59]
Tribolium castaneum Tenebrionidae N [89]
Cry8Ga Holotrichia oblita Scarabaeidae N [60]
Holotrichia parallela Scarabaeidae N [60]
Cry8Ka Anthonomus grandis Curculionidae A 2.83–8.93 [63]
Cry8Na Anomala corpulenta Scarabaeidae N [65]
Holotrichia oblita Scarabaeidae N [65]
Holotrichia parallela Scarabaeidae A 3.18 × 10 × 1010 CFU/g [65]
Cry8Sa Holotrichia serrata (F.) Scarabaeidae A [145]
Cry9Bb Diabrotica undecimpuntata Chrysomelidae N [146]
Diabrotica virgifera Chrysomelidae N [146]
Leptinotarsa decemlineata Chrysomelidae N [146]
Anthonomus grandis Curculionidae N [146]
Cry9Da Anomala cuprea Scarabaeidae A [77]
Tribolium castaneum Tenebrionidae N [89]
Cry10Aa Anthonomus grandis Curculionidae A 7.12 [78]
Cry14Aa Tribolium castaneum Tenebrionidae LA [89]
Cry15Aa Leptinotarsa decemlineata Chrysomelidae N [147]
Cry18Aa1 Melontha melontha Scarabaeidae A [148]
Cry22Aa Anthonomus grandis Curculionidae A 0.75 μg/well [68]
Tribolium castaneum Tenebrionidae A 1.25 g/10 g [89]
Cry22Ab Cylas brunneus Brentidae A 1.01 μg/g [56]
Cylas puncticollis Brentidae A 0.78 μg/g [56]
Diabrotica virgifera Chrysomelidae A 39.4 μg/cm2 [69]
Diabrotica undecimpuntata Chrysomelidae N [69]
Leptinotarsa decemlineata Chrysomelidae N [69]
Anthonomus grandis Curculionidae A 3.12 μg/well [68]
Cry22Ba Diabrotica virgifera Chrysomelidae N [68]
Anthonomus grandis Curculionidae A [68]
Cry23Aa/37Aa Cylas brunneus Brentidae A 0.46 μg/g [56]
Cylas puncticollis Brentidae A 0.42 μg/g [56]
Acanthoscelides obtectus Chrysomelidae A [75]
Anthonomus grandis Curculionidae A [149]
Popillia japonica Scarabaeidae A [37]
Tribolium castaneum Tenebrionidae A 6.30 μg SC/μL [37,61]
Cry34Aa Diabrotica virgifera Chrysomelidae N [40]
Cry34Ab Diabrotica undecimpuntata Chrysomelidae LA [150]
Diabrotica virgifera Chrysomelidae N [40,82]
Cry34Ac Diabrotica virgifera Chrysomelidae N [40]
Cry34Aa/35Aa Diabrotica undecimpuntata Chrysomelidae A 34.1 μg/well [151]
Diabrotica virgifera Chrysomelidae A 34 μg/cm2 [81,151]
Cry34Ab/35Ab Rhyzophertha dominica Bostrichidae N [17]
Diabrotica undecimpuntata Chrysomelidae A [150]
Diabrotica virgifera Chrysomelidae A 3 μg/cm2 [40,81]
Oryzaephilus surinamensis Cucujidae LA [17]
Sitophilus oryzae Curculionidae LA [17]
Trogoderma variabile Dermestidae N [17]
Tenebrio molitor Tenebrionidae LA [17]
Tribolium castaneum Tenebrionidae LA [17]
Tribolium castaneum Tenebrionidae N [96]
Cry34Ac/35Ac Diabrotica virgifera Chrysomelidae A 7 μg/cm2 [40,81]
Cry34Ba/35Ba Diabrotica virgifera Chrysomelidae A [39]
Cry35Aa Diabrotica virgifera Chrysomelidae N [40]
Cry35Ab Diabrotica virgifera Chrysomelidae N [40,82]
Cry35Ac Diabrotica virgifera Chrysomelidae N [40]
Cry36A Diabrotica virgifera Chrysomelidae A 147.3 μg/well [151]
Cry37Aa Tribolium castaneum Tenebrionidae A 1.25 g/10 g [89]
Cry38Aa Diabrotica virgifera Chrysomelidae N [39]
Cry43Aa Anomala cuprea Scarabaeidae A [152]
Cry43Ba Anomala cuprea Scarabaeidae N [152]
Cry51Aa Diabrotica undecimpuntata Chrysomelidae N [79]
Diabrotica virgifera Chrysomelidae N [79]
Leptinotarsa decemlineata Chrysomelidae A [79]
Tribolium castaneum Tenebrionidae A 1.45 g/10 g [89]
Cry55Aa Phyllotreta cruciferae Chrysomelidae A [80]
Tribolium castaneum Tenebrionidae N [89]
Cyt1Aa Chrysomela scripta F Chrysomelidae A 132.6 [72]
Cyt2Ca Diabrotica undecimpuntata Chrysomelidae A 25 μg/well [83]
Diabrotica virgifera Chrysomelidae A 10.8 μg/well [83]
Leptinotarsa decemlineata Chrysomelidae A [83]
Diapepes abbreviatus Curculionidae A 50.7 [84,85]
Popillia japonica Scarabaeidae A [83]
Tribolium castaneum Tenebrionidae A [83]

(a) The parameter is mortality. A = active; N = not active; LA = low activity, with significant inhibition of growth; (b) LC50 = lethal concentration that causes 50% mortality of the insects. Data are expressed in μg/mL, unless otherwise stated. “//” separate two different values of the LC50.