Table 1.
Linear regression parameters of solvent and matrix-matched calibration curves for seventeen toxin analogues. Matrix effect was evaluated as %ME calculated according to Equation (1) for each toxin: %ME = 100—no signal effect, %ME < 100—signal suppression, %ME > 100—signal enhancement. Italic values represent calibration range for TTX. Calibration curves of each toxin that visualize the signal suppression/enhancement can be found in Supplementary Material Figure S1.
| Table | Calibration Range | Correlation Coefficient (R2) | Matrix Effect (ME) | ||
|---|---|---|---|---|---|
| nmol/L | µg STX eq/kg µgTTX/kg |
Solvent | Matrix-Match | %ME | |
| dcSTX | 4.1–130.0 | 21.0–671.5 | 0.995 | 0.998 | 59 |
| dcNEO | 1.9–60.8 | 5.25–167.9 | 0.991 | 0.995 | 69 |
| STX | 4.1–132.6 | 24.8–793.7 | 0,996 | 0.997 | 65 |
| NEO | 4.1–130.2 | 25.7–821.2 | 0.991 | 0.996 | 81 |
| TTX, 4-epiTTX | 4.9–157.2 | 31.4–1003.3 | 0.993 | 0.999 | 45 |
| 11-deoxyTTX | 0.2–5.0 | 0.9–30.3 | 0.997 | 0.998 | 182 |
| 4,9-anhydroTTX | 0.6–19.8 | 3.4–119.2 | 0.996 | 0.998 | 169 |
| dcGTX3 | 0.7–21.6 | 3.8–120.1 | 0.998 | 0.999 | 75 |
| GTX6 | 2.0–64.5 | 0.6–19.8 | 0.996 | 0.997 | 76 |
| GTX5 | 6.2–200.2 | 2.8–88.8 | 0.998 | 0.999 | 86 |
| C2 | 1.8–58.8 | 2.0–64.5 | 0.997 | 0.999 | 59 |
| GTX2 | 3.6–114.4 | 20.2–649.0 | 0.998 | 1.000 | 98 |
| GTX3 | 1.1–36.0 | 12.9–412.7 | 0.998 | 0.999 | 81 |
| GTX1 | 6.4–205.2 | 29.4–942.2 | 0.997 | 0.999 | 102 |
| GTX4 | 2.7–87.0 | 6.5–207.3 | 0.990 | 0.998 | 93 |
| dcGTX2 | 3.7–117.0 | 20.3–649.0 | 0.996 | 0.999 | 73 |
| C1 | 0.8–25.0 | 0.67–21.6 | 0.999 | 0.999 | 51 |