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. 2020 Jul 1;12(7):432. doi: 10.3390/toxins12070432

Figure 3.

Figure 3

Effect of protease on the antimicrobial activity of the cell-free supernatant from L. taiwanensis. Proteinase K (100 µg/mL) was added to the cell-free supernatant (77.1 μg peptides/mL, MIC50 against S. Gallinarum) from L. taiwanensis. The 15% SDS-PAGE was performed to identify the degradation of proteins and peptides (a), and the proteinase K-treated sample was used to examine the antimicrobial activity against S. Gallinarum (b). The arrow in (a) represents degraded proteins or peptides. The y-axis and different letters (A, B and C) in the graphs represent the relative growth of S. Gallinarum and significant differences (p < 0.05), respectively.