Figure 5.

Antibacterial activity of various fractions from L. taiwanensis cell-free supernatant. (a) The supernatants, obtained after the centrifugation of 30%, 50%, or 70% methanol extracts (M30 to M70) from the cell-free supernatant, were used to examine the antimicrobial activity against S. Gallinarum, whose MIC50 was determined. (b) The solution, loaded to Sep-Pak column, was used to perform flow-through (FT), followed by washing twice (WT) with 0.05% trifluoroacetic acid solution, and eluted by 10% to 80% acetonitrile (CAN), and finally the fractions were also used to determine the MIC50. The y-axis and different letters (A, B, C, D and E) in the graphs represent the MIC50 and significant differences (p < 0.05), respectively.