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. 2020 Jul 1;12(7):432. doi: 10.3390/toxins12070432

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antibacterial activity of Taiwanencins identified from the L. taiwanensis genome. Tan 1, 2, and 3 were synthesized as peptides comprising the amino acid sequences FAWKNRRKIQEVLNHGQS (Tan 1), IVGGVLNLAGGALIMAAGIVKLASKTK (Tan 2), and VGAVSLIAWLAMWV (Tan 3), respectively, which were further examined for their antimicrobial activity against a Gram-negative bacterium (EPEC) (a) and a Gram-positive bacterium (B. cereus) (b). The y-axis and different letters (A, B, C, D, E, a, b and c) in the graphs represent the relative growth of pathogenic bacteria and significant differences (p < 0.05), respectively. (c) Scanning electron micrographs of EPEC treated with synthetic Tan 2 (5 mg/mL). EPEC was treated without (the left image) or with synthetic Tan 2 for 16 h (the right image). The red arrows indicate the pores forming in the EPEC membrane.

Antibacterial activity of Taiwanencins identified from the L. taiwanensis genome. Tan 1, 2, and 3 were synthesized as peptides comprising the amino acid sequences FAWKNRRKIQEVLNHGQS (Tan 1), IVGGVLNLAGGALIMAAGIVKLASKTK (Tan 2), and VGAVSLIAWLAMWV (Tan 3), respectively, which were further examined for their antimicrobial activity against a Gram-negative bacterium (EPEC) (a) and a Gram-positive bacterium (B. cereus) (b). The y-axis and different letters (A, B, C, D, E, a, b and c) in the graphs represent the relative growth of pathogenic bacteria and significant differences (p < 0.05), respectively. (c) Scanning electron micrographs of EPEC treated with synthetic Tan 2 (5 mg/mL). EPEC was treated without (the left image) or with synthetic Tan 2 for 16 h (the right image). The red arrows indicate the pores forming in the EPEC membrane.