Figure 7.

Identification of claustrum PN subtypes. A, Silhouette analyses were performed on data at several hierarchical levels to identify the optimal number of subclusters. The highest silhouette width (SI width) was obtained at hierarchical level 5, containing six clusters. B, SI width of single neurons within each of the six subclusters was ranked; the high SI widths of neurons in subclusters PN1 and PN3 indicates clearest separation from other subclusters. Negative values for SI widths of some PN2 cells suggests the presence of further subclusters. One of the clusters was a single VIP-IN (circled). C, Dendrogram of PN subtypes. D–F, Alternative methods of cell clustering, based on PC (D), t-SNE (E), and LLE (F). All approaches revealed subclusters similar to those generated by hierarchical clustering, confirming the presence of five PN subtypes plus an errant VIP-IN. Same color scheme is used to identify individual neurons in B–D.