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. 2020 Jul 23;5:178. [Version 1] doi: 10.12688/wellcomeopenres.16031.1

Figure 1.

Figure 1.

( A) Gene model of SULT-OR ( Smp_089320), indicating the position of the two exons, one intron, and UTRs, spanning 4837 bp on the reverse strand of chromosome 6. Boxes filled in dark red represent the protein coding sequence. Schistosoma mansoni (PRJEA36577). Assembly: Smansoni_v7 (GCA_000237925.3). Region: Scaffold SM_V7_6:3,183,084-3,188,924. Adapted from WormBase ParaSite 14 31. ( B) Nucleotide sequence of the start of exon 1 indicating location and sequence of gRNA target site, predicted double-stranded break (DSB), protospacer adjacent motif (PAM), and the PvuII restriction site. ( C) Reference PCR amplicon, showing the positions of the gRNA, PAM, DSB, forward and reverse PCR primers, and forward and reverse sequence reads, as well as a SNP site found in many sequence reads. The diagrams are drawn to scale.