FIGURE 1.

Structure of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) nucleocapsid dimerization domain. (a) Domain structure of the SARS‐CoV‐2 nucleocapsid protein, as defined previously, ⁴⁶ , ⁴⁷ with plot showing the Jalview alignment conservation score (three‐point smoothed; gray) ⁶¹ and DISOPRED3 disorder propensity (red) ⁶² for nine related coronavirus N proteins (SARS‐CoV, SARS‐CoV‐2, Middle‐East respiratory syndrome [MERS]‐CoV, HCoV‐OC43, HCoV‐HKU1, HCoV‐NL63, and HCoV‐229E, infectious bronchitis virus [IBV], and Murine Hepatitis virus [MHV]). SR, serine/arginine rich domain; SB, spacer B. The boundary between SB and N3 is not well defined due to low identity between SARS‐CoV/SARS‐CoV‐2 and MHV N proteins. ⁴⁷ All purified truncations are noted at bottom. (b) Top‐down view of the SARS‐CoV‐2 N_2b dimer, with one monomer colored as a rainbow (N‐terminus blue, C‐terminus red) and the other colored white. See Figure S1a for comparison with other structures of this domain. (c) Structural overlay of the SARS‐CoV‐2 N_2b dimer (blue) and the equivalent domain of SARS‐CoV‐N (PDB ID 2CJR) ³⁴