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. 2020 Jul 29;8:622. doi: 10.3389/fcell.2020.00622

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Copyright © 2020 Sheel, Shao, Brown, Johnson, Hamilton, Sun, Oppenheimer, Smith, Visconti, Markstein, Bigelow and Schwartz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of signal transduction genes during ISM development. (A) RNA-seq analysis of Eclosion Hormone Receptor (EHR) and Ecdysis Triggering Hormone Receptors (ETHR) A and B across ISM development. RNA-seq analysis of EHR (blue), ETHR-A (red), and ETHR-B (green). EHR was significantly induced on day 18 (p < 0.001, n = 3 independent replicates for each stage of development). (B) Expression of cGMP-dependent protein kinase (PKG) (blue) and cAMP-dependent protein kinase (red) across the ISMs across development. PKG was induced during development (p = 0.002, n = 3 independent replicates for each stage of development).