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. 2020 Jul 29;8:622. doi: 10.3389/fcell.2020.00622

FIGURE 5.

FIGURE 5

The role of Acheron and BBH1 on ecdysial muscle death in Drosophila. (A) Data from Flybase modENCODE Tissue Expression Data based on RNA-seq analysis (CG17386). Acheron is transiently induced ∼600-fold just before adult eclosion. (B) Percentage of Drosophila that eclosed successfully in control and Acheron RNAi expressing animals. Acheron RNAi blocked eclosion (p < 0.0001, mean ± SE, n = 28 and 202, respectively). (C) Pre-eclosion pharate adult transgenic flies expressing red fluorescent protein (RFP) in the skeletal muscles of control (top) and Acheron (CG17386) RNAi flies. (D) Fluorescent imaging of the same animals showing that Acheron RNAi knockdown led to the precocious death of the ptilinal muscles. (E) Percentage of flies that eclosed successfully in control and BBH1 RNAi animals (p = 1.0, mean ± SE, n = 28 and 154, respectively). (F) Fluorescent image of a control fly head 36 h post-eclosion showing the normal loss of the ptilinal muscles. (G) Fluorescent image of a BBH1 RNAi fly head 36 h post-eclosion showing the retention of the ptilinal muscles. Note that the muscles in the proboscis (bottom front of the heads) persist in the adult. wt, wild type. Scale bars = 100 μm.