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Expression of cytochrome c in the ISMs. Cytochrome c immunohistochemical staining of ISMs demonstrates abundant punctate staining in the ISMs prior to adult eclosion (A), which was lost throughout the tissue following eclosion (B). The nuclei are stained blue. Scale bar = ∼280 μm. (C) Western blot analysis of Acheron and cytochrome c in the ISMs before and after adult eclosion.
