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. Author manuscript; available in PMC: 2020 Aug 5.
Published in final edited form as: Genesis. 2020 Jun 16;58(7):e23369. doi: 10.1002/dvg.23369

Fig 4. EmGFP+ particles are detectable in plasma from ECCD63-emGFP mice and they co-localize with EV tetraspanins.

Fig 4.

(A) Zetaview analysis of mouse plasma detects two peaks: one representing total circulating EVs and a smaller, left-shifted peak representing EC-derived, CD63emGFP+ EVs. (B) Quantification of total circulating EVs (emGFP−) versus EC-derived EVs (emGFP+) in control versus tumor-bearing mice, n=3 mice per group. Data are presented as mean +/− SEM. (C) Exoview analysis of mouse plasma isolated from ECCD63-emGFP mice. Capture antibodies are indicated on the x-axis. Data are presented as mean +/− SEM. (D) CD63 antibody capture of EVs using Exoview analysis. (E) CD63 antibody capture using Exoview shows absence of CD45 detection in CD63emGFP+ EVs. (F) Representative plots showing detection and sorting of emGFP particles in serum using FACS in ECCD63-emGFP reporter mice. (G) qPCR analysis of EC-selective miRNAs in the emGFP− (non EC-derived) and emGFP+ (EC-derived) particles.