Figure 1.

Ocular perfusion protocol. (Left) Representative pressure versus time trace during a perfusion. After an acclimatization period of 30 minutes at 13 mm Hg, the pressure is reduced to 12 mm Hg, at which point the perfusion is considered to begin (t = 0). Pressure is then increased in eight steps. The center panel illustrates the corresponding inflow versus time. (Right) Overview of the discrete volume method used to determine ocular volume at each pressure increment. The blue shaded region represents aqueous humor outflow, and the overlying brown shaded region indicates the flow involved in increasing the ocular volume. For each pressure step, the area of this region indicates the total volume increase (system plus eye), from which ocular compliance can be calculated.