Table 7.
Expression of Seven Stretch-Responsive Protein-Coding Genes Examined with ddPCR
| RNA-Seq | ddPCR | ||||
|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Fold Change | P | Fold Change | P |
| ACAT2 | Acetyl-coenzyme A acetyltransferase | 2.43 | 9.42E-03 | 2.51 | 3.41E-03 |
| ACSS2 | Acetyl-coenzyme A synthetase | 2.07 | 4.27E-02 | 2.78 | 1.97E-02 |
| DHCR7 | 7-Dehydrocholesterol reductase | 2.21 | 4.45E-03 | 2.65 | 1.46E-02 |
| EBP | 3-β-Hydroxysteroid-δ(8),δ(7)-isomerase | 2.20 | 2.48E-03 | 2.97 | 3.74E-03 |
| NSDHL | Sterol-4-α-carboxylate 3-dehydrogenase | 2.24 | 3.62E-03 | 2.72 | 4.09E-03 |
| GPER1 | G-protein coupled estrogen receptor 1 | 3.17 | 2.73E-02 | 0.96 | 9.89E-01 |
| PFN2 | Profilin-2 | –2.11 | 1.69E-04 | 1.07 | 9.52E-01 |
These genes were related to steroid metabolism and/or were central hubs in the mRNA–miRNA interaction analysis. The differential expression of five of the seven mRNAs was validated by ddPCR.