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. Author manuscript; available in PMC: 2020 Aug 5.
Published in final edited form as: Cell Rep. 2020 Jun 23;31(12):107815. doi: 10.1016/j.celrep.2020.107815

Figure 2. SLP76 Is Required for CD28-Mediated LLPC Survival.

Figure 2.

(A and B) Purified PCs from BM (A) or SP (B) of WT or SLP76-KI mice were treated with control Ig or anti-CD28 mAb-coated beads for 16 h in serum-free media, and viability was assessed using trypan blue exclusion. Representative of two independent experiments. *p < .05.

(C) Total numbers of CD3+ T cells and PCs from three individual WT or age-matched SLP76-KI mice were quantified using FACS. Representative of two independent experiments. *p < .05.

(D) BM and SP MNCs from three WT or age-matched SLP76-KI mice were stained for CD138, B220, and CD3. Percentage BMPCs or SPPCs on the basis of gating B220−CD138+ population. Data represent two independent experiments, with the percentage PCs for six individual mice quantified. ***p < 0.001.

(E) BM MNCs were harvested from four WT or SLP76-KI mice and total IgG-secreting cells quantified using ELISpot. Data are representative of two independent experiments. ***p < 0.001.

Statistical analysis by Student’s t test. Error bars represent ± SD.