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. 2020 May 18;61(5):29. doi: 10.1167/iovs.61.5.29

Figure 2.

Figure 2.

Statins suppress TGF-β2-mediated induction of MMP-2 and MMP-9 in astrocytes from human ONH. Astrocytes were incubated with simvastatin (A, D), lovastatin (B, E), and atorvastatin (C, F) (5 µg/mL) for 1 hour, followed by TGF-β2 (5 ng/mL) for 24 hours. Proteins in conditioned medium were assessed by western blotting. Representative immunoblots (A–C) and quantitation (D–F) show that TGF-β2 induced MMP-2 and MMP-9 approximately 1.5- to 2-fold. Prior incubation with statins similarly suppressed the TGF-β2-mediated induction of MMP-2 and MMP-9 to basal levels. Equivalent gel loading was determined by Coomassie Brilliant Blue staining. Data are shown as fold changes relative to DMSO controls. Values were compared between TGF-β2 with and without statins (*P < 0.05, †P < 0.01 vs. DMSO; n = 3).