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. 2020 May 19;61(5):33. doi: 10.1167/iovs.61.5.33

Figure 2.

Figure 2.

Protein expression and TEER of AAP cells treated with ABCA1 agonist GW3965 for 24 hours. (A) Western blot shows that ABCA1 protein expression was upregulated in the GW3965-treated group compared with the blank and vehicle treated groups (n = 3 cell lines). (B, C) Western blot detected reduced Cav1 and increased eNOS after GW3965 treatment (n = 3 cell lines). (D) Griess assay showed significantly higher NO concentration in the GW3965-treated group than vehicle group control (n = 3 cell lines). (E) TEER of the AAP monolayer declined notably in the GW3965-treated group compared with the vehicle group (n = 4 cell lines). C1, blank; C2, vehicle; C3, GW3965. ***P < 0.001, **P < 0.01, compared with vehicle group, one-way ANOVA. Results are expressed as mean ± standard deviation. GADPH, glyceraldehyde 3-phosphate dehydrogenase.