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AAP cells transduced with ABCA1–shRNA encoding lentivirus. Bright-field (A) and fluorescence (B) micrographs of AAP cells transduced with shRNA sequences specifically targeting ABCA1 transcript (ABCA1–shRNA). Green fluorescence indicates successful transduction in the cell. (C) ABCA1 transcript accumulation was 0.56-fold lower in response to ABCA1–shRNA than in response to scrambled shRNA transduced controls. **P < 0.05. Data were analyzed by one-way ANOVA. Scale bar = 100 µm.
