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. 2020 May 19;61(5):33. doi: 10.1167/iovs.61.5.33

Figure 4.

Figure 4.

Cav1 and eNOS expression in AAP cells transduced with ABCA1–shRNA lentivirus. (A, B) Western blot showed upregulated Cav1 and downregulated eNOS in ABCA1–shRNA transduced AAP cells (n = 3 cell lines). (C) Griess assay showed that NO concentration was significantly lower in the ABCA1–shRNA transduced AAP cells compared with scrambled control transduced AAP cells (n = 3 cell lines). (D) TEER of AAP monolayer was notably higher in the ABCA1–shRNA transduced cells compared with scrambled shRNA transduced cells (n = 3 cell lines). A1, blank; A2, scrambled shRNA; A3, ABCA1–shRNA. *P < 0.05, ** P < 0.01 compared with scrambled shRNA groups. A, B, and C were analyzed using one-way ANOVA. D was analyzed using the Mann-Whitney U test.