The mRNA levels of fibrotic mediators in OFs. Go (n = 6) and non-GO (n = 4) OFs were treated with 10 ng/mL TGF-β1 for 24 hours in the absence or presence of pretreating with 100 µg/mL Gyps for 24 hours. The expression of HAS2 (A), COL1A2 (B), α-SMA (C), and FN1 (D) mRNA in each cell group was evaluated by RT-qPCR. **P < 0.01, ***P < 0.001, and ****P < 0.0001 versus TGF-β1-stimulated cells, 1-way ANOVA.