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. 2020 May 27;61(5):62. doi: 10.1167/iovs.61.5.62

Figure 2.

Figure 2.

HCF on HC-HA/PTX3 Form Aggregates, Revert to Keratocytes, and Resist to TGFβ1. Passage 3 HCF were seeded on plastic with or without immobilized HA or HC-HA/PTX3 for 7 days (A, Bar = 100 µm). Transcript expression of keratocan (B) and TGFβs and TGFβRs (D) was determined at day 1 by quantitative RT-PCR using the expression level on plastic set as 1 (*P < 0.05, ***P < 0.001, n = 3). Expression of keratocan protein was determined at Day 2 and Day 7 by Western blot using β-actin as the loading control (C). HCF seeded on plastic with or without immobilized HA or HC-HA/PTX3 in DMEM+10% FBS were switched to DMEM+ITS for 24 hours before being added with 10 ng/mL TGFβ1 for 24 hours before immunostaining of pSmad2/3 and for 72 hours before immunostaining to α-SMA (E, nuclear staining by Hoechst 33342, Bar = 100 µm).