Figure 2.

Temporal expression of collagen XII in wild-type mice. (A) Col12a1 mRNA expression was higher during stromal development but was maintained in the adult WT stromas. Relative Col12a1 mRNA expression was obtained by real-time PCR analysis using a comparative ΔCT method that used β-actin as an internal control and sample 1 of a WT day 4 cornea as the reference sample. The sample means and standard deviations were calculated from three or four corneal stromas of different mice. (B) Collagen XII protein was present in the maturing and mature stroma. Representative Wes image shows that the short form and long form of collagen XII were present and accumulated throughout the corneal development stages. Total protein was used as a protein loading control. The right-side panel shows the relative collagen XII protein quantitation that was normalized to total protein. The standard deviation was obtained by Wes analysis of two sets of independent protein samples.