KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-His6 | Abcam | Cat# Ab18184; RRID:AB_444306 |
| Sheep anti-mouse IgG:HRP | Biorad | Cat# AAC10P; RRID:AB_321929 |
| Goat polyclonal anti-GST | GE Healthcare | Cat# 27-4577-01; RRID:AB_771432 |
| Donkey anti-goat IgG:HRP | Santa Cruz Biotech | Cat# Sc-2020; RRID:AB_631728 |
| Bacterial and Virus Strains | ||
| LOBSTR BL21(DE3) Competent Cells | Kerafast | Cat# EC001 |
| One Shot BL21(DE3) Competent Cells | Invitrogen | Cat# C600003 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| His6 BFL-1ΔC WT, C55S, C4S/C19S, C4S/C19S/C55S | Walensky Lab | N/A |
| GST MCL-1ΔNΔC | Walensky Lab | N/A |
| GST BCL-XLΔC | Walensky Lab | N/A |
| Complete Protease Inhibitor Tablet Cocktail | Sigma-Aldrich | Cat# 16829800 |
| IPTG | Gold Biotechnology | Cat# I2481C |
| β-mercaptoethanol (BME) | Thermo Fisher Scientific | Cat# PI35602 |
| 4E14 | Walensky Lab | N/A |
| 4E14-2-thiopyridine | Walensky Lab | N/A |
| D-4E14 | Walensky Lab | N/A |
| 5-Me-4E14 | Walensky Lab | N/A |
| D-Nal-4E14 | Walensky Lab | N/A |
| Disulfide Tethering Fragment Library | Wells Lab | N/A |
| Rink Amide AM Resin 200-400 mesh | Novabiochem | Cat# 855004 |
| Glutaric Anhydride | Sigma Aldrich | Cat# G3806 |
| N,N-Dimethylformamide | Pharmco | Cat# zh324UVHPLC |
| N,N-Diisopropylethylamine | Sigma-Aldrich | Cat# 387649 |
| Dichloromethane | Sigma-Aldrich | Cat# D65100 |
| HATU | ApexBio Tech LLC | Cat# A7022 |
| Cysteamine Hydrochloride | Sigma Aldrich | Cat# M6500 |
| Tris(2-carboxyethyl)phosphine hydrochloride | Sigma Aldrich | Cat# C4706 |
| Aldrithiol-2 | Sigma Aldrich | Cat# 143049 |
| Bis(2-dimethylaminoethyl) Disulfide Dihydrochloride | TCI | Cat# B2272 |
| Critical Commercial Assays | ||
| Q5 Site Directed Mutagenesis Kit | New England Biolabs | Cat# E0554S |
| Rat/Mouse Cytochrome c Quantikine ELISA Kit | R&D Systems | Cat# MCTC0 |
| Deposited Data | ||
| BFL-1/4E14 Crystal Structure | This Paper | PDB: 6VO4 |
| BFL-1/D-NA-NOXA SAHB Crystal Structure | Harvey et al., 2018 | PDB: 5WHH |
| Apo BFL-1 Crystal Structure | Harvey et al., 2018 | PDB: 5WHI |
| BFL-1/NOXA BH3 Crystal Structure | Protein Data Bank | PDB: 3MQP |
| Recombinant DNA | ||
| Plasmid PET17b | Novagen | Cat# 69663 |
| Plasmid PET17b_BFL1ΔC_WT | Walensky Lab | N/A |
| Plasmid PET17b_BFL1ΔC_C4S/C19S | Walensky Lab | N/A |
| Plasmid PET17b_BFL1ΔC_C4S/C19S/C55S | Walensky Lab | N/A |
| Plasmid PET17b_BFL1ΔC_C55S | Walensky Lab | N/A |
| Plasmid PGEX-4T-1 | GE Healthcare | Cat# 28-9545-49 |
| Plasmid PGEX-4T-1_MCL-1ΔNΔC | Walensky Lab | N/A |
| Plasmid PGEX-4T-1_BCL-XLΔC | Walensky Lab | N/A |
| Software and Algorithms | ||
| GraphPad Prism | Graphpad Software Inc. | https://www.graphpad.com/scientific-software/prism/ |
| XIA2 | Winter, 2010 | https://xia2.github.io |
| POINTLESS | Evans, 2006 | http://www.ccp4.ac.uk |
| XDS | Kabsch, 2010 | http://xds.mpimf-heidelberg.mpg.de/ |
| Phaser | McCoy et al., 2007 | https://www.phenix-online.org/documentation/reference/phaser.html |
| Phenix | Adams et al., 2010 | https://www.phenix-online.org/ |
| Coot | Emsley and Cowtan, 2004 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Schrodinger Maestro | Schrodinger, Version 2016-2 | Schrodinger, LLC |
| Pymol | The PyMol Molecular Graphics System, Version 1.7.4.0 | Schrodinger, LLC |
| ProteinLynx Global Server (PLGS) 3.0.1 | Waters Corporation | http://www.waters.com/waters/en_US/ProteinLynx-Global-SERVER-(PLGS)/nav.htm?cid=513821&locale=en_US |
| DynamX 3.0 | Waters Corporation | http://www.waters.com/waters/library.htm?cid=511436&lid=134832928&locale=en_US |
| Other | ||
| Superdex 75 10/300 GL size exclusion column | GE Healthcare Life Sciences | Cat# 29148721 |