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. 2020 Jul 29;10:1218. doi: 10.3389/fonc.2020.01218

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Copyright © 2020 Greville, Llop, Huang, Creagh-Flynn, Pfister, O'Flaherty, Madden, Peracaula, Rudd, McCann and Saldova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes seen in UPLC N-glycan profiles, are associated with the mRNA expression levels of glycosyltransferases. Quantitative RT-PCR gene expression analyses were undertaken for selected glycosyltransferases (MGAT5 and ST3GAL4) and enzymes involved in the sugar nucleotide donor pathway (MPI, TSTA3, and GMDS) following the culturing of cells in differential hypoxic conditions (0.5–2% O₂) compared to normoxia (21% O₂) controls. The relative expression level of each gene was calculated according to the ddCt normalized with TATA-Box Binding Protein (TBP). Each condition was undertaken on one biological replicate (n = 1) and three technical replicates (n = 3).