Table 1.
Summary of glycomic changes in all cell lines.
| Significantly altered glycans in hypoxia | A2780 | A2780cis | PEO1 | PEO4 | MB-231 | MB-436 |
|---|---|---|---|---|---|---|
| A2F1G1 | ||||||
| M5A1G1 | ||||||
| FA2BG2, FA3G2 | ||||||
| M7 | ||||||
| A2G2S1, A3G3 | ||||||
| M8, FA2F2G2 | ||||||
| M8 | ||||||
| M5A2BF1G2Lac1S(6)1 | ||||||
| A2G2S2 | ||||||
| A2G2S2*-isomer of higher GU value | ||||||
| FA2G2S2 | ||||||
| A3G3S2 | ||||||
| A3G3S3 | ||||||
| A3F1G3KDN3 | ||||||
| FA3BG3S4 | ||||||
| Glycan features | ||||||
| Oligomannose | ||||||
| Hybrids | ||||||
| Bisecting glycans (B) | ||||||
| Lacs with outer arm fucose | ||||||
| Monoantennary glycans (A1) | ||||||
| Tetraantennary glycans (A4) | ||||||
| Monogalactosylated glycans (G1) | ||||||
| Monosialylated glycans (S1) | ||||||
| Disialylated glycans (S2) | ||||||
Increased glycans are in red and decreased in green.
Any changes seen in hypoxia are summarized and highlighted (from 0.5 to 2%).
Structure abbreviations: All N-glycans have two core GlcNAcs; F at the start of the abbreviation indicates a core-fucose α1,6-linked to the inner GlcNAc; Mx, number (x) of mannose on core GlcNAcs; Ax, number of antenna (GlcNAc) on trimannosyl core; A2, biantennary with both GlcNAcs as β1,2-linked; A3, triantennary with a GlcNAc linked β1,2 to both mannose and the third GlcNAc linked β1,4 to the α1,3 linked mannose; A4, GlcNAcs linked as A3 with additional GlcNAc β1,6 linked to α1,6 mannose; B, bisecting GlcNAc linked β1,4 to β1,3 mannose; Gx, number (x) of β1,4 linked galactose on antenna; F(x), number (x) of fucose linked α1,3 to antenna GlcNAc; Sx, number (x) of sialic acids linked to galactose. Where not specified, the glycan contains sialic acid linked α2,3 and α2,6.