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. Author manuscript; available in PMC: 2020 Aug 5.
Published in final edited form as: Cell Rep. 2015 Mar 5;10(9):1599–1613. doi: 10.1016/j.celrep.2015.02.014

Figure 5. Blockade of ErbB2/Akt Signaling Pathway Inhibits Tumor Growth Induced by Pten/Smad4 Deficiency.

Figure 5.

(A) Two chamber invasion assays of Beas-2B cells with knockdown of PTEN and SMAD4 (siSMAD4/PTEN) and the treatment of kinase inhibitors as indicated.

(B) Quantification of the results (A) using one-way ANOVA (p value is *p < 0.05, **p < 0.01, ***p < 0.01).

(C–G) Inhibition of tumor formation in 9-month-old Ptend/dSmad4d/d mice the treatment of kinase inhibitors. Representative whole-lung images and H&E staining of lung tissues in WT and Ptend/dSmad4d/d mice are shown in (C). Quantitative analysis of tumor growth in Ptend/dSmad4d/d mice with or without the treatment of kinase inhibitors is shown in (D)–(G). Statistical analysis was performed using one-way ANOVA (p value is *p < 0.05, **p < 0.01, ***p < 0.01) on the ratio of whole lung to whole body and using Student’s t test (p value is **p < 0.01) on the maximal diameter and the number of surface tumors. Black arrows indicate lung tumors.