Figure 3. ROS production in whole zebrafish larvae.
Zebrafish embryos were exposed to several concentrations of xenobiotics from 6 hpf to 96 hpf. At 96 hpf, larvae were plated into a 96 well plate, and ROS production was measured via H2DCFDA after stimulation with PMA. Maximum fluorescence of each well was used for all analyses; results of two replicate experiments are reported. (A) Acenaphthenequinone, (B) azathioprine, (C) benzo[a[pyrene, (D) dexamethasone, (E) dichloroacetic acid, (F) 17-β-estradiol, (G) hydroquinone, (H) lead (II) acetate trihydrate, (I) methoxychlor, (J) phenanthrene, (K) tributyltin oxide and (L) trichloroethylene. Bis I (10 μM; selective protein kinase C inhibitor) was used as a positive control. Significance (*p < 0.05) was determined by a one-way ANOVA with Dunnett’s post-hoc test for pairwise comparisons to the DMSO control. Red data points denote significance for tested chemicals.