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. 2020 Aug 5;15(8):e0237095. doi: 10.1371/journal.pone.0237095

Fig 1. Cell contraction and preparation of C2C12 myotube electrical pulse stimulation-conditioned media (EPS-CM).

Fig 1

(A) AMPK phosphorylation (Thr172) level in C2C12 myotubes by 3h EPS. (B) Lactate dehydrogenase activity in the conditioned media. (C, D) Lactic acid and glucose concentration in the conditioned media. The initial concentration of glucose in the medium was 5.5 mM. (E, F) Immunoblotting of IL-6 and IL-15 in the conditioned media. Data are expressed as mean ± SEM (n = 8 (A-E), n = 4 (F)). **; p < 0.01. N.S., not significant.