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. 2020 Aug 5;15(8):e0237095. doi: 10.1371/journal.pone.0237095

Fig 4. Effects of myotube EPS-CM treatment on adiponectin secretion of 3T3-L1 adipocytes.

Fig 4

(A) 3T3-L1 adipocytes were continuously treated with 50% myotube EPS-CM, and on day 10 post-differentiation, cell lysates were subjected to immunoblotting for adiponectin. (B) Following the final 48 h treatment with myotube EPS-CM (day 8–10), the levels of adiponectin in the culture media of 3T3-L1 adipocytes were determined using ELISA. Data are expressed as mean ± SEM (n = 12 (A), n = 8 (B)). *; p < 0.05. **; p<0.01.