Table 2. The difference between the four study groups in standardized Ct value (ΔCt) for miRNA-155, miRNA 146b, miRNA 146a and miRNA 203.
| Study groups | Mir-155 | Mir-146b | Mir-146a | Mir-203 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Range | Mean | SD | SE | Range | Mean | SD | SE | Range | Mean | SD | SE | Range | Mean | SD | SE | ||
| Diabetes | healthy periodontium (n = 10) | 3.38–8.54 | 6.05 | 1.84 | 0.58 | 6.58–9.81 | 7.99 | 1.14 | 0.36 | 9.02–12.43 | 10.19 | 1.04 | 0.33 | 2.87–9.17 | 6.68 | 1.99 | 0.63 |
| periodontitis (n = 14) | 2.68–8.49 | 5.23 | 1.8 | 0.48 | 3.85–11.88 | 6.9 | 2.12 | 0.57 | 8.19–14.98 | 12.28 | 1.98 | 0.53 | 1.49–10.54 | 7.09 | 2.62 | 0.7 | |
| Non-diabetics | healthy periodontium (n = 14) | 6–15.54 | 10.1 | 2.68 | 0.72 | 7.96–18.45 | 11.91 | 2.97 | 0.79 | 9.04–18.24 | 13.78 | 2.59 | 0.69 | 6.14–13.01 | 8.99 | 1.97 | 0.53 |
| Periodontitis (n = 15) | 2.15–10.73 | 6.32 | 2.38 | 0.61 | 5.74–12.31 | 8.29 | 2 | 0.52 | 7.02–15.11 | 11.67 | 2.43 | 0.63 | 0.84–10.62 | 6.43 | 2.71 | 0.7 | |
| P (ANOVA) | <0.001 | <0.001 | 0.002 | 0.029 | |||||||||||||
| Effect of diabetes (Diabetes with healthy periodontium vs. non-diabetic with healthy periodontium | |||||||||||||||||
| ΔΔCt | -4.05 | -3.92 | -3.58 | -2.3 | |||||||||||||
| Fold change (2-ΔΔCt) | 16.5 ↑ | 15.1 ↑ | 12 ↑ | 4.9 ↑ | |||||||||||||
| P value | <0.001 | <0.001 | <0.001 | 0.024 | |||||||||||||
| Effect of periodontitis among diabetics (Diabetic with unhealthy periodontium vs. diabetic with healthy periodontium) | |||||||||||||||||
| ΔΔCt | -0.83 | -1.09 | 2.09 | 0.41 | |||||||||||||
| Fold change (2-ΔΔCt) | 1.8 ↑ | 2.1 ↑ | 0.24 ↓ | 0.75 ↓ | |||||||||||||
| P value | 0.38 [NS] | 0.24 [NS] | 0.024 | 0.68 [NS] | |||||||||||||
| Effect of periodontitis among non-diabetics (Non-diabetic with unhealthy periodontium vs. non-diabetic with healthy periodontium) | |||||||||||||||||
| ΔΔCt | -3.77 | -3.62 | -2.1 | -2.56 | |||||||||||||
| Fold change (2-ΔΔCt) | 13.7 ↑ | 12.3 ↑ | 4.3 ↑ | 5.9 ↑ | |||||||||||||
| P value | <0.001 | <0.001 | 0.012 | 0.006 | |||||||||||||
| Effect of diabetes combined with periodontitis (diabetic/ unhealthy periodontium x non-diabetic/ healthy periodontium) | |||||||||||||||||
| Delta Delta Ct | -4.87 | -5.01 | -1.50 | -1.89 | |||||||||||||
| Fold change (2^-delta delta Ct) | 29.3↑ | 32.2↑ | 2.8↑ | 3.7↑ | |||||||||||||
| P value | <0.001 | <0.001 | 0.07[NS] | 0.041 | |||||||||||||
| effect of diabetes on patients with periodontitis (diabetic/ unhealthy periodontium x non diabetic/ unhealthy periodontium) | |||||||||||||||||
| Delta Delta Ct | -1.10 | -1.39 | 0.60 | 0.67 | |||||||||||||
| Fold change (2^-delta delta Ct) | 2.1↑ | 2.6↑ | 0.7↓ | 0.6↓ | |||||||||||||
| P value | 0.19[NS] | 0.1[NS] | 0.46[NS] | 0.46[NS] | |||||||||||||
[NS]: non-significant difference.
↑ over-expressed, ↓ under-expressed.
Ct: Cycle threshold.
SD: Standard Deviation.
SE: Standard error.