Table 1.
Characteristics of testicular (intracytoplasmic sperm injection using testicular sperm) group and ejaculate (intracytoplasmic sperm injection cycle using ejaculated sperm) group (first control)
| Parameter | T-ICSI (n=52) | Ej-ICSI (control, n=87) | P |
|---|---|---|---|
| Age (year), mean±s.d. | |||
| Male | 38.9±5.9 | 37.0±6.6 | 0.051 |
| Female | 34.4±3.7 | 33.5±4.8 | 0.3 |
| Female body mass index (kg m−2), mean±s.d. | 23.0±3.4 | 24.0±4.8 | 0.87 |
| Type of infertility, n (%) | |||
| Primary | 36 (69.2) | 62 (71.3) | 0.8 |
| Secondary | 16 (30.8) | 25 (28.7) | |
| Serum FSH level (IU l−1), mean±s.d. | 6.8±7.2 | 7.5±7.7 | 0.85 |
| Total testosterone (nmol l−1), mean±s.d. | 12.0±4.0 | 13.0±5.4 | 0.24 |
| Right testicular volume (ml), mean±s.d. | 18.0±2.7 | 17.0±3.6 | 0.13 |
| Left testicular volume (ml), mean±s.d. | 17.0±2.4 | 16.0±3.6 | 0.11 |
| Spermogram, mean±s.d. | |||
| Volume (ml) | 3.1±1.7 | 3.0±1.5 | 0.55 |
| Concentration (×106 ml−1) | 22.9±31.6 | 35.0±47.0 | 0.09 |
| Total motility (%) | 42.0±23.9 | 48.3±20.9 | 0.06 |
| Normal morphology (%) | 1.9±1.7 | 2.3±1.5 | 0.13 |
| DFI (%) | 37.6±15.9 | NA | NA |
| A+B | 0.27±0.20 | 0.45±0.26 | <0.001 |
| Number of oocytes, mean±s.d. | |||
| Retrieved | 11.2±5.6 | 12.6±5.9 | 0.17 |
| MII | 10.3±5.3 | 10.1±5.0 | 0.74 |
| Fertilization rate, mean±s.d. | 0.58±0.27 | 0.72±0.21 | 0.004 |
| Cycles with failed embryo development, n (%) | 4/52 (7.7) | 8/87 (9.2) | 1.0 |
| Number of total embryos, mean±s.d. | 6.2±4.1 | 7.0±3.6 | 0.51 |
DFI: DNA fragmentation index; MII: metaphase II; FSH: follicle-stimulating hormone; ICSI: intracytoplasmic sperm injection; T-ICSI: ICSI using testicular sperm; Ej-ICSI: ICSI cycle using ejaculated sperm; s.d.: standard deviation; A+B: progressive motility; NA: not available