Skip to main content
. 2020 Jun 18;5(12):e136852. doi: 10.1172/jci.insight.136852

Figure 2. SLIT3 deficiency reduces fibrillar collagen production in vivo.

Figure 2

(A) Masson’s trichrome–stained histological sections of lung, spleen, and kidney in 8-week-old Slit3−/− and WT mice. Results are representative of samples obtained from 5 animals per genotype group. Scale bars: 100 μm, 60 μm, and 100 μm from top to bottom. (B) Transcript levels of Slit3, Col1a1, and Col3a1 in the aortic adventitia, lung, spleen, and kidney in 8-week-old Slit3−/− and WT mice (n = 4–6 per group). (C) Linear regression analysis between the transcript levels of Slit3 and Col1a1 in the aortic adventitia, lung, spleen, and kidney from 8-week-old WT mice (n = 5–7 per group). (D) Quantification of tissue collagen content by assessment of hydroxyproline concentrations in the femur, skin, lung, spleen, and kidney in 8-week-old Slit3−/− and WT mice (n = 5–8 per group). Data are presented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. WT mice using the unpaired 2-tailed Student’s t test (B and D). n, number; M, male; F, female.